Which of the following events occurred during the reign of Alexander the Great?

The establishment of the port of Alexandria during the reign of Alexander the Great is a significant historical milestone that reflects his contributions to urban development and cultural integration. Founded in 331 BC, Alexandria became one of the most important cities of the ancient world and served as a center for trade, culture, and scholarship. Its strategic location on the Mediterranean coast facilitated commerce and helped to spread Greek culture throughout the region after Alexander's conquests.

Alexandria also became home to the famous Library of Alexandria, which symbolized the blending of Greek and Eastern knowledge. This reflects Alexander's broader vision of uniting different cultures under his empire. The foundation of Alexandria was pivotal in shaping the Hellenistic world, which thrived even after Alexander's death, emphasizing the long-lasting impact of his reign on the subsequent cultural and political landscape.

The other events listed, while significant in their own right, did not take place during Alexander's reign or are not as directly associated with his influence as the establishment of Alexandria is.